Technical Specifications

Product Overview

Applications Cancer
Stem Cell/Regenerative Medicine
Tissue Modeling
Co-culture
Drug Toxicity Screening
Product Specifications Format:
Porosity:
Thickness:
Materials:
Well Plate Disc; 10″x10″ sheets
8-15 microns
0.5mm
PET, Cellulose Acetate
Cell Types Currently Used Cancer Cell Lines:
Fibroblasts:
Endothelial Cells:
Stem Cells:
Other:
MDA-MB-231, MCF-7, A549
NIH/3T3, HFL1
HUVEC, EAhy926
Primary MSCs
HEK293
In Vitro Models Stem Cell:
Tumorogenesis:
Chondrogenesis, Osteogenesis
Fibroblast, Endothelial and Tumor Co-culture
Compatible Assays Proliferation:
Microscopy:
RNA:
Immunohistochemistry:
Subculturing
Protein Extraction
AlamarBlue, MTT
SEM, TEM, Optical, Fluorescence, Confocal
TRIzol, Spin Column, In Situ Hybridization
Cryosectioning, Immunostaining
Applications

Cancer
Stem Cell/Regenerative Medicine
Tissue Modeling
Co-culture
Drug Toxicity Screening

Product Specifications

Format: Well Plate Disc; 10″x10″ sheets
Porosity: 8-15 microns
Thickness: 0.5mm
Materials: PET, Cellulose Acetate

Cell Types Currently Used

Cancer Cell Lines: MDA-MB-231, MCF-7, A549
Fibroblasts: NIH/3T3, HFL1
Endothelial Cells: HUVEC, EAhy926
Stem Cells: Primary MSCs
Other: HEK293

In Vitro Models

Stem Cell: Chondrogenesis, Osteogenesis
Tumorogenesis: Fibroblast, Endothelial and Tumor Co-culture

Compatible Assays

Proliferation: AlamarBlue, MTT
Microscopy: SEM, TEM, Optical, Fluorescence, Confocal
RNA: TRIzol, Spin Column, In Situ Hybridization
Immunohistochemistry: Cryosectioning, Immunostaining, Subculturing, Protein Extraction

Protocols

Protocol HTML Version PDF Version
Cell Seeding HTML PDF
Fluorescence Staining HTML PDF
Cryosection Preparation HTML PDF
Proliferation
– AlamarBlue HTML PDF
– CellTiter96 HTML PDF
RNA Isolation HTML PDF
SEM Preparation HTML PDF
All Protocols PDF